biotinylated human trka (Carna Inc)
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Structured Review
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biotinylated human trka
Biotinylated Human Trka, supplied by Carna Inc, used in various techniques. Bioz Stars score: 95/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+human+trka/TRKA/pmc13100123-287-0-7
Average 95 stars, based on 23 article reviews
Biotinylated Human Trka, supplied by Carna Inc, used in various techniques. Bioz Stars score: 95/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+human+trka/TRKA/pmc13100123-287-0-7
Average 95 stars, based on 23 article reviews
biotinylated human trka - by Bioz Stars,
2026-11
95/100 stars
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1) Product Images from "Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis"
Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis
Journal: Nature Communications
doi: 10.1038/s41467-026-70000-6
Figure Legend Snippet: a Structure of PF-07245303. b PF-07245303 co-crystal structure with ITK protein identifying binding sites and docking mode to ITK ATP catalytic site at 1.35 Å (PDB 9NWX). c PF-07245303 binding kinetics to recombinant ITK, TRKA, TRKB, and TRKC protein using SPR. d In vitro absorption, distribution, metabolism, and excretion profile for PF-07245303. e Kinome selectivity for PF-07245303 tested at 1 µM against a panel of 218 kinase constructs colored by percent inhibition in biochemical assays using 1 mM ATP.
Techniques Used: Binding Assay, Recombinant, In Vitro, Construct, Inhibition
Figure Legend Snippet: Effects of PF-07245303 on the kinase activity of human a TRKA, b TRKB, and c TRKC were determined. Graphs of mean % inhibition ± SD at each concentration a n = 5 experiments and b , c n = 4 experiments. PF-07245303 inhibits d TRKA, e TRKB, and f TRKC-dependent phosphorylation in cells expressing human TRKA, TRKB or TRKC and p75. Ligand-induced receptor activation is detected by receptor phosphorylation-induced functional complementation and restored β-galactosidase activity measured by chemiluminescence. Inhibition of d TRKA, e TRKB, and f TRKC activation with PF-07245303 or selective pan-TRK inhibitor PF-06273340. d – f Graph of mean % inhibition ± SD at each concentration across n = 4 experiments. g Determination of PF-07245303 inhibition of TRKA in human blood basophils. Graph of mean % inhibition ± SD of NGF-induced increase in the percent of basophils double positive for CD123 and CD203c at each concentration of PF-07245303 or PF-06273340 across n = 5 donors. h NGF-induced TRKA phosphorylation was measured in human skin sections treated with PF-07245303 (10–0.1 μM) or with 10 μM of PF-06273340 and stimulated with NGF. Representative results for phospho-TRKA activation and mean % inhibition ± SD in skin from one donor. Skin from 3 donors with 6 replicates per condition was evaluated. Comparisons of mean differences = Δ versus control is done by t-test with 113 degrees of freedom, and FDR-adjusted two-sided p -values. 0.1 μM has a Δ = −26.5 a t = −2.28 and p -value = 0.0244. 0.3 μM has a Δ = − 49.8 a t = −4.78 and p -value = 6.58e-06. One micromolar has a Δ = −74.6 a t = −7.18 and p = 1.34e-10. Three micromolar has a Δ = −86.6 a t = −8.33, and p = 5.44e-13. Ten micromolar has a Δ = −93.2 a t = −8.96 and p = 3.91e-14. Notable changes in phospho-TRKA levels relative to vehicle were defined as p < 0.0001 and F < −0.50 and denoted with ***. F = 2 −estimated ΔΔCt treatment difference and F and F 95% CI are in Supplementary Table .
Techniques Used: Activity Assay, Inhibition, Concentration Assay, Phospho-proteomics, Expressing, Activation Assay, Functional Assay, Control
Related Articles
Binding Assay:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Recombinant:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( In Vitro:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Construct:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Inhibition:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Activity Assay:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Concentration Assay:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Phospho-proteomics:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Expressing:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Activation Assay:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Functional Assay:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Control:Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis Article Snippet: The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.The captured ITK was then cross-linked with a 30 s injection of 1:1 pre-mixed NHS/EDC, followed by 30 s of ethanolamine (amine coupling kit, Cytiva), resulting in a level of captured and cross-linked ITK protein ranging from 7K–10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis. Article Snippet: The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.The captured ITK was then cross-linked with a 30s injection of 1:1 pre-mixed NHS/EDC, followed by 30s of ethanolamine (amine coupling kit, Cytiva) resulting in a level of captured and cross-linked ITK protein ranging from 7K – 10K RU.. Biotinylated human TRKA, TRKB, and TRKC proteins ( |
